Escherichia coli is often used as a hygiene indicator for fecal contamination. Poultry meat products play a pivotal role as transmitters of sporadic campylobacteriosis cases in the EU.
jejuni represents the major reported species. With 220,682 confirmed human cases in 2019, Campylobacter infections remain the most common bacterial gastro-intestinal disease in the European Union. are frequent fecal contaminants with serious implications for human health. We conclude that v-qPCR can improve food safety under the consideration of some limitations.Īlong the poultry production chain, Thermotolerant Campylobacter spp. coli might be a complementary hygiene indicator. Interestingly, both bacteria showed similar kinetics of inactivation upon the application of reduction strategies, suggesting that E. v-qPCR analysis detected bacterial survival upon the application of lactic acid, which awaits further analysis. The effect of treatment with peroxyacetic acid on survival was consistently detected by CFU and v-qPCR. The results showed that freezing and storage in cool conditions are potentially underestimated by CFU but not by v-qPCR. The short- and long-term effects of decontaminating chicken skin on the survival of both C. coli targeting the uidA gene, encoding β-glucuronidase, which is usually detected for phenotypic species identification. We developed a viability real-time PCR (v-qPCR) for the quantification of viable E. Therefore, colony forming units (CFU) may underestimate the infection risk of pathogens. Stress exposure may transiently cease bacterial division. spp., the bacterium is generally present in the avian gut. coli is considered as hygiene indicator since, in contrast to Campylobacter. are fecal contaminants of chicken meat with serious implications for human health. Supports Dynamic Volume which includes Simple Volume, Spanned Volume, Stripe Volume, Mirror Volume and Raid Volume.Thermotolerant Campylobacter spp.
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